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mgieasy whole genome bisulfite library preparation kit  (Complete Genomics Inc)


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    Complete Genomics Inc mgieasy whole genome bisulfite library preparation kit
    Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
    Mgieasy Whole Genome Bisulfite Library Preparation Kit, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mgieasy+whole+genome+bisulfite+library+preparation+kit/MGIEasy+Whole+Genome+Methylation+Sequencing+Library+Prep+Kit+V3%2E0/pm40470697-341-6-12
    Average 97 stars, based on 4 article reviews
    mgieasy whole genome bisulfite library preparation kit - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis."

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    doi: 10.1002/advs.202500058

    Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated samples. Whole-genome methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
    Figure Legend Snippet: Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated samples. Whole-genome methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.

    Techniques Used: Genome Wide, DNA Methylation Assay, Methylation

    Related Articles

    Genome Wide:

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis.
    Article Snippet: Target fragments were size-selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.Target fragments were size-selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.. Library construction was performed using an MGIEasy whole-genome bisulfite library preparation kit (MGI).. After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high-density DNA nanoball chip and sequenced using circumferential probe-anchored polymerase amplification on a DNBSEQ-G400 sequencer (BGI) with PE150 sequencing.After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high-density DNA nanoball chip and sequenced using circumferential probe-anchored polymerase amplification on a DNBSEQ-G400 sequencer (BGI) with PE150 sequencing.

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar ‐Modified Arabidopsis
    Article Snippet: Target fragments were size‐selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.Target fragments were size‐selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.. Library construction was performed using an MGIEasy whole‐genome bisulfite library preparation kit (MGI).. After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high‐density DNA nanoball chip and sequenced using circumferential probe‐anchored polymerase amplification on a DNBSEQ‐G400 sequencer (BGI) with PE150 sequencing.After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high‐density DNA nanoball chip and sequenced using circumferential probe‐anchored polymerase amplification on a DNBSEQ‐G400 sequencer (BGI) with PE150 sequencing.

    DNA Methylation Assay:

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis.
    Article Snippet: Target fragments were size-selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.Target fragments were size-selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.. Library construction was performed using an MGIEasy whole-genome bisulfite library preparation kit (MGI).. After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high-density DNA nanoball chip and sequenced using circumferential probe-anchored polymerase amplification on a DNBSEQ-G400 sequencer (BGI) with PE150 sequencing.After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high-density DNA nanoball chip and sequenced using circumferential probe-anchored polymerase amplification on a DNBSEQ-G400 sequencer (BGI) with PE150 sequencing.

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar ‐Modified Arabidopsis
    Article Snippet: Target fragments were size‐selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.Target fragments were size‐selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.. Library construction was performed using an MGIEasy whole‐genome bisulfite library preparation kit (MGI).. After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high‐density DNA nanoball chip and sequenced using circumferential probe‐anchored polymerase amplification on a DNBSEQ‐G400 sequencer (BGI) with PE150 sequencing.After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high‐density DNA nanoball chip and sequenced using circumferential probe‐anchored polymerase amplification on a DNBSEQ‐G400 sequencer (BGI) with PE150 sequencing.

    Methylation:

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis.
    Article Snippet: Target fragments were size-selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.Target fragments were size-selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.. Library construction was performed using an MGIEasy whole-genome bisulfite library preparation kit (MGI).. After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high-density DNA nanoball chip and sequenced using circumferential probe-anchored polymerase amplification on a DNBSEQ-G400 sequencer (BGI) with PE150 sequencing.After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high-density DNA nanoball chip and sequenced using circumferential probe-anchored polymerase amplification on a DNBSEQ-G400 sequencer (BGI) with PE150 sequencing.

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar ‐Modified Arabidopsis
    Article Snippet: Target fragments were size‐selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.Target fragments were size‐selected using 2% agarose gel electrophoresis, recovered using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), and amplified using polymerase chain reaction to create libraries.. Library construction was performed using an MGIEasy whole‐genome bisulfite library preparation kit (MGI).. After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high‐density DNA nanoball chip and sequenced using circumferential probe‐anchored polymerase amplification on a DNBSEQ‐G400 sequencer (BGI) with PE150 sequencing.After quality checking, the library was amplified using phi29 to generate DNA nanoballs, which were loaded onto a high‐density DNA nanoball chip and sequenced using circumferential probe‐anchored polymerase amplification on a DNBSEQ‐G400 sequencer (BGI) with PE150 sequencing.



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    mgieasy whole genome bisulfite library preparation kit  (Complete Genomics Inc)
    97
    Complete Genomics Inc mgieasy whole genome bisulfite library preparation kit
    Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
    Mgieasy Whole Genome Bisulfite Library Preparation Kit, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mgieasy+whole+genome+bisulfite+library+preparation+kit/MGIEasy+Whole+Genome+Methylation+Sequencing+Library+Prep+Kit+V3%2E0/pm40470697-341-6-12
    Average 97 stars, based on 1 article reviews
    mgieasy whole genome bisulfite library preparation kit - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated samples. Whole-genome methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.

    Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis.

    doi: 10.1002/advs.202500058

    Figure Lengend Snippet: Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated samples. Whole-genome methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.

    Article Snippet: Library construction was performed using an MGIEasy whole-genome bisulfite library preparation kit (MGI).

    Techniques: Genome Wide, DNA Methylation Assay, Methylation